Microfabricated Devices for Sizing DNA and Sorting Cells
نویسندگان
چکیده
We have microfabricated devices to size and sort microscopic biological objects, ranging from cells to single molecules of DNA. Sizing is accomplished by fluorescent excitation and detection. The devices are fabricated in a silicone elastomer using a replica method. Single molecules of DNA have been sized to 10% accuracy, and manipulation of E. Coli cells has been demonstrated.
منابع مشابه
Disposable Microdevices for Dna Analysis and Cell Sorting
We have developed microfabricated devices to size and sort microscopic objects, based on measurement of fluorescent properties. With these devices, we have demonstrated sizing and sorting on various biological entities, ranging from E. Coli cells to single molecules of DNA. The microfabricated devices have several advantages over macroscopic systems, including size, cost and sensitivity. For ex...
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